Content Determination of Berberine, Epiberberine, Coptisine and Palmatine in Shutongantai Capsules by HPLC
10.3969/j.issn.1005-5304.2014.07.018
- VernacularTitle:HPLC测定舒通安泰胶囊中小檗碱、表小檗碱、黄连碱、巴马汀含量
- Author:
Jianzhong HOU
;
Lijian YU
;
Cheng YANG
- Publication Type:Journal Article
- Keywords:
Shutongantai Capsules;
HPLC;
berberine;
epiberberine;
coptisine;
palmatine;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(7):61-63
- CountryChina
- Language:Chinese
-
Abstract:
Objective To build a method for determination of berberine, epiberberine, coptisine and palmatine in Shutongantai Capsules by HPLC.Methods Thermo C18 column (5μm, 4.0 mm× 250 mm) was used with acetonitrile-0.05 mol/L potassium dihydrogen phosphate solution (50∶50, 0.4 g sodium dodecyl sulfate was added in each 100 mL solution, to adjust the pH 4.0 with phosphoric acid) as mobile phase;the flow rate was 1.0 mL/min;detection wavelength was 345 nm;column temperature was 30℃.Results Berberine hydrochloride showed a good linear relationship in the range of 0.180 34-0.901 68μg (r=0.999 8).Conclusion The method is simple, accurate and reproducible, and can be used for the quality control of Shutongantai Capsules.
