Differentiation of odontoblast-like cells from human exfoliated deciduous teeth-derived stem cells by transforming growth factor β3 in combination with heparin in vitro
10.3969/j.issn.1006-5725.2014.12.010
- VernacularTitle:转化生长因子β3联合肝素在人乳牙牙髓干细胞向成牙本质样细胞分化中的作用
- Author:
Fei REN
;
Hui ZHOU
;
Jianping LIU
;
Jincai ZHANG
;
Pingping XU
;
Qin YANG
;
Xiaochun CHEN
- Publication Type:Journal Article
- Keywords:
Stem cells from human exfoliated deciduous teeth;
Transforming growth factor β3;
Heparin;
Odontoblast-like cells
- From:
The Journal of Practical Medicine
2014;(12):1887-1890
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the ability of human exfoliated deciduous teeth-derived stem cells (SHED) to differentiate into odontoblast-like cells. Methods SHEDs were isolated by enzyme digestion method. The 3nd passage SHEDs were incubated with 25 ng/mL recombinant human TGF-β3 , or with TGF-β3 in combination with heparin. The DSPP expression was detected by Q-PCR and Western-blotting assay. Alizarin red staining, immunhistochemistry assay and alkaline phosphatase(AKP) activity assay were performed, respectively. Result The AKP activity was enhance by TGF-β3 in combination with heparin. Alizarin red staining was positive in TGF-β3-heparin groups, with the increase of DSPP expression at both mRNA and protein level. Conclusion TGF-β3 in combination with heparin can enhance the differentiation of human exfoliated deciduous teeth-derived stem cells into odontoblast-like cells.
