Methylation state of MEG3 gene in epithelial ovarian cancer and its clinical significanc research

VernacularTitle:上皮性卵巢癌组织中MEG3基因异常甲基化状态及其临床意义
Author: Qin ZHAO ; Jianqi LI ; Yanqing ZHOU ; Linyu TAN ; Juan LI ; Xiujie SHENG
Publication Type:Journal Article
Keywords: Ovarian neoplasms; MEG3; Methylation
From: The Journal of Practical Medicine 2014;(12):1902-1905
CountryChina
Language:Chinese
Abstract: Objective To study the relationship between the methylation status of CPG islands in MEG3 gene promoter region of epithelial ovarian cancer and its clinical and pathological features. Methods The promoter methylation status was evaluated by MSP (methylation-specific polymerase chain reaction ) in 47 cases of ovarian cancer tissue and 15 cases of normal control. Results The methylation ratio (42.6%) of the MEG3 genes in the ovarian cancer was statistically significantly higher (P = 0.035 ) than that (13.3%) in the normal control. The methyation rate of the group with an age > 60 years old was slightly higher than that of the group with an age≤60 years old, without statistically significant (P > 0.05), so was observed in ovarian cancers of stage Ⅰ andⅡ than that in stage Ⅲ and Ⅳ. There were also no significant differences in MEG3 gene methylation positive rate neither in different pathological grading nor in various ovarian cancer tissues (P > 0.05). Conclusion Abnormal methylation in MEG3 gene may be associated with epithelial ovarian cancer , but no relation to its clinical pathology.