Effects of lipoxin receptor agonist BML-111 on IFN-βand IE86 mRNA expression of macrophages infected by human cytomegalovirus

VernacularTitle:脂氧素受体激动剂BML-111对巨细胞病毒感染后巨噬细胞表达IFN-β及IE86 mRNA的影响
Author: Xiaohong CHEN ; Sainan SHU ; Xinglou LIU ; Hui WANG ; Ju ZHANG ; Xiaoyi DU ; Ge LI ; Feng FANG
Publication Type:Journal Article
Keywords: Cytomegalovirus; Macrophage; Lipoxin; Type 1 interferon
From: The Journal of Practical Medicine 2014;(13):2055-2057
CountryChina
Language:Chinese
Abstract: Objectives To investigate the effects of lipoxin receptor agonist BML-111 on IFN-βand IE86 mRNA expression of macrophages infected by human cytomegalovirus (HCMV). Methods Macrophages were infected with HCMV (MOI=0.5), and the cultured cells were randomly divided into control group, HCMV group, HCMV+BML-111 group, and HCMV+MP group. The cells were collected at 0,1,2,4,8 and 12 h after infection, and the levels of IFN-βand IE86 mRNA were tested by real-time PCR. Results Compared with HCMV group, the levels of IFN-βmRNA in HCMV+BML-111 group increased significantly (P < 0.05), while the levels of IFN-βmRNA in HCMV+MP group decreased significantly (P < 0.05); Compared with HCMV group, there were no significant differences of the levels of IE86 mRNA in HCMV+BML-111 group (P>0.05), while the levels of IE86 mRNA in HCMV+MP group increased significantly (P < 0.05). Conclusion BML-111 exerts antiviral activity by promoting the expression of IFN-βmRNA at the early stage of HCMV infection.