Determination of Bisphenolic and Halogenated Bisphenolic Compounds in Human Urine by High Performance Liquid Chromatography-Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.140069
- VernacularTitle:固相萃取液相色谱串联质谱法检测尿中双酚类和卤代双酚类物质
- Author:
Xiaojian HU
;
Haijing ZHANG
;
Xiaohong WANG
;
Changming DING
;
Yinlong JIN
;
Shaobin LIN
- Publication Type:Journal Article
- Keywords:
Bisphenolic compounds;
Halogenatedbisphenolic compounds;
Human urine;
Solid phase extraction;
High performance liquid chromatography-tandem mass spectrometry
- From:
Chinese Journal of Analytical Chemistry
2014;(7):1053-1056
- CountryChina
- Language:Chinese
-
Abstract:
A method was developed for the determination of four kinds of bisphenolic and halogenated bisphenolic compounds including bisphenol F, bisphenol A, tetrachlorobisphenol A, tetrabromobisphenol A in human urine using high performance liquid chromatography-tandem mass spectrometry. The analytes was extracted by solid phase extraction. The separation of the analytes was achieved on an Atlantis T3 column (3. 0×150 mm, 3 μm) gradient eluted with the mobile phase of acetonitrile and water at the rate of 250 μL/min, and detected by an electrospray ionization tandem mass spectrometry in the multiple-reaction-monitoring negative mode. The quantification was carried out by matrix-matched calibration curve. The average recoveries at 3 spiked levels were 86%-118%, with intra-day precision of 2 . 6%-17 . 0% and inter-day precision of 3. 2%-18. 0%. The limits of detection of four analytes (S/N=3) were 0. 01-0. 25 μg/L. The method was applied to the analysis of 200 human urines samples and the results showed that the method was simple, sensitive and reliable.
