Rapid Identification of Multiple Bacteria on a Microfluidic Chip
10.3724/SP.J.1096.2014.31222
- VernacularTitle:微流控芯片快速鉴定多重细菌
- Author:
Xiaoxia WEN
;
Banglao XU
;
Weixin WANG
;
Guangtie LIANG
;
Bin CHEN
;
Yinmei YANG
;
Dayu LIU
- Publication Type:Journal Article
- Keywords:
Microfluidic chip;
Bacteria identification;
Multiplex chromogenic reaction;
Rapid detection
- From:
Chinese Journal of Analytical Chemistry
2014;(6):791-798
- CountryChina
- Language:Chinese
-
Abstract:
We developed a microfluidic device to integrate sample introduction, bacteria culturing and results reading. The identification of multiple bacteria was achieved by combining the spatial resolution of the arrayed bacteria culture chambers and the color resolution benefited from the bacteria specific chromogenic media. A set of 4 common pathogenic bacteria responsible for urinary tract infection were used as a model to test the microfluidic assay. Our results showed that the bacteria identification assay can be completed in 15 h, with a limit of detection (LOD) of bacteria density down to 10 cfu / mL. Clinical sample testing using the microchip approach showed a coincidence rate of 96. 3% as compared with the conventional method. The developed microfluidic approach is simple and rapid, thus hold the potential to serve as a powerful tool for detection of multiple bacteria.
