Preparation and Application of Podophyllotoxin Sensor Based on Molecularly Imprinting Technique
10.3724/SP.J.1096.2014.40017
- VernacularTitle:鬼臼毒素分子印迹电化学传感器的研制及应用
- Author:
Chunqiong WANG
;
Huiping BAI
;
Jing CHEN
;
Qiue CAO
- Publication Type:Journal Article
- Keywords:
Podophyllotoxin;
Molecularly imprinted membrane;
Electrochemical sensor;
In situ polymerization
- From:
Chinese Journal of Analytical Chemistry
2014;(6):842-846
- CountryChina
- Language:Chinese
-
Abstract:
A novel electrochemical sensor for the determination of podophyllotoxin (PPT) was prepared by modifying a PPT-imprinted membrane, which was synthesized by in situ polymerization with PPT as template, methacrylic acid ( MAA) as functional monomer and ethylene glycol dimethacrylate ( EGDMA) as cross linking agent at the molar ratio of PPT/ MAA/ EGDMA as 1 : 2 : 100, on glassy carbon electrode ( GCE) followed by eluting PPT from the modified GCE with methanol/ acetic acid (1: 1, V/ V). The properties of the electrochemical sensor modified with PPT-imprinted membrane were characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS). Under the optimum conditions, it was found that the response of peak currents was linear to the concentration of PPT in range of 1. 0 - 120 μmol/ L with the detection limit of 0. 47 μmol/ L. The sensor not only showed high selectivity, but also exhibited good stability and reproducibility. The sensor was satisfactorily applied to the determination of PPT in podophyllum hexandrum and human serum samples with the relative standard deviation (RSD) below 3. 9% and recovery ranging from 95. 0% to 103. 3% .