Determination of alkylglycerol contents in breast milk
10.3969/j.issn.1000-3606.2014.06.010
- VernacularTitle:母乳中烷氧基甘油含量的测定
- Author:
Linxi QIAN
;
Huanlei SONG
;
Tao ZHENG
;
Yan ZHONG
;
Wenjuan YU
;
Shengmei WU
;
Wei CAI
- Publication Type:Journal Article
- Keywords:
breast milk;
alkylglycerol;
gas chromatography
- From:
Journal of Clinical Pediatrics
2014;(6):540-543
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine alkylglycerol (AKG) contents and variation in breast-milk of lactating women. Methods Five cases of healthy lactating women with term delivery were selected from June 2011 to June 2012. Breast-milk samples were collected at 1, 2, 3, 4, 8, 12, 16, 20 and 24 weeks postpartum. Breast milk samples were extracted, saponificated and derivatized. AKGs composition in breast-milk was quantitatively analyzed by GC chromatography. Results Mean 16C:0 AKG content in breast-milk decreased from(17.31 ± 3.59)× 10-3g/L to(11.14 ± 1.83)× 10-3g/L. Mean 18C:0 AKG content de-creased from(14.95±6.00)×10-3g/L to(9.68±2.51)×10-3g/L. Mean 18C:1 AKG content fluctuated between(4.64±0.91)×10-3g/L and(3.95±0.68)×10-3g/L. Conclusions 16C:0, 18C:0 and 18C:1 AKG contents exist in Chinese breast-milk through determina-tion by GC chromatography, and the concentrations vary among different stages of lactation.
