Magnetic Affinity Immunoassay Based Enzyme-Labeled Phage Displayed Antibody

VernacularTitle:基于酶标噬菌体抗体的磁分离免疫分析方法
Author: Xihui MU ; Zhaoyang TONG ; Qibin HUANG ; Bing LIU ; Zhiwei LIU ; Lanqun HAO ; Jinping ZHANG
Publication Type:Journal Article
Keywords: Enzyme-labeled phage displayed antibody; Magnetic affinity immunoassay; β-Bungarotoxin
From: Chinese Journal of Analytical Chemistry 2014;(6):785-790
CountryChina
Language:Chinese
Abstract: A new magnetic affinity immunoassay (MAIA) strategy based on enzyme-labeled phage displayed antibody was developed. The assay consisted of a sandwich format in which immobilized polyclonal antibody (pcAb) on magnetic microparticle was used for capture probe, and enzyme-labeled phage displayed antibody for specific detection probe to increase enzyme amount and enhance detection signal. By the proposed method,β-bungarotoxin (β-BGT) was successfully detected. A linear relationship between absorbance value and the concentration of β-BGT in the range of 0. 016-62. 5 μg / L was obtained. The linear regression equation was Y=0. 641X+1. 355 (R =0. 9925, n = 13, p<0. 0001) with a detection limit of 0. 016 μg / L. In comparison with the traditional ELISA, this method gave a 10-fold better sensitivity in β-BGT detection. This strategy also gave a 4-fold better sensitivity comparing with the MAIA based on enzyme labeled monoclonal antibody (mcAb). Due to low detection limit, acceptable reproducibility and high specificity, this method holds great promise in toxin trace detection.