Analysis of microsatellite polymorphism in Candida albicans strains isolated from patients with vulvo-vaginal candidiasis in Guizhou region
10.3760/cma.j.issn.0254-5101.2014.06.005
- VernacularTitle:贵州地区感染外阴阴道念珠菌病的白念珠菌微卫星多态性研究
- Author:
Meizhu WANG
;
Yu CAO
;
Xiaoshan XU
;
Peifang ZHANG
;
Lili MOU
;
Fengyan BAI
;
Yingqian KANG
- Publication Type:Journal Article
- Keywords:
Single-strand conformation polymorphism;
Candida albicans;
Genotype
- From:Chinese Journal of Microbiology and Immunology
2014;(6):431-434
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genotype distribution of microsatellite locus CAI among Candida albicans ( C.albicans ) strains and to evaluate its relationship with the epidemic of vulvovaginal candidiasis ( VVC) in Guizhou region .Methods Ninety independent C.albicans strains isolated from pa-tients with VVC in Guizhou were investigated based on single-strand conformation polymorphisms ( SSCP ) and GeneScan analysis .The genotypes of C.albicans strains were identified by microsatellite locus CAI pol-ymorphism analysis .The gene polymorphism and the cluster of C.albicans strains were analyzed by using software SPSS 19.0.A logistic regression model was used to analyze the relationship between genotype distri -bution of CAI microsatellite among C.albicans strains and VVC infection .Results Twenty-seven distinct CAI genotypes with various patterns were identified from 90 C.albicans strains by GeneScan analysis .Clus-ter analysis showed that the C.albicans strains were classified into three clusters ( ClusterⅠto Cluster Ⅲ) . Three predominant genotypes including 30-45, 32-46 and 30-46 and other 7 highly similar genotypes be-longed to clusterⅡthat accounted for 70.0%(63 strains) in all strains.The odds ratio for the predominant genotypes associated with VVC infection was 4.3.Conclusion The predominant distribution of genotypes was observed among the isolated C.albicans strains.The predominant genotypes of C.albicans were highly associated with the occurrence of VVC .
