Study on the antigenicity of Streptococcus pneumonia polysaccharide, its derivatives and conjugates by three immunological assays
10.3760/cma.j.issn.0254-5101.2014.06.013
- VernacularTitle:3种免疫学方法对肺炎球菌结合物及其中间品的多糖抗原性研究
- Author:
Fanglei LIU
;
Yali HOU
;
Bing WU
;
Jia LIU
;
Xinru WANG
;
Xubo YU
;
Yanhong MU
;
Aiping LIU
;
Zhiqiang ZHAO
;
Guilin XIE
- Publication Type:Journal Article
- Keywords:
Streptococcus pneumococcal conjugate;
Inhibition ELISA;
Antigenicity;
Double immu-nodiffusion;
Rate nephelometry
- From:
Chinese Journal of Microbiology and Immunology
2014;(6):471-475
- CountryChina
- Language:Chinese
-
Abstract:
Objective To monitor and analyze the antigenicity of Streptococcus pneumonia polysac-charide, its derivatives and conjugates by three immunological assays .Methods Inhibition ELISA and rate nephelometry(RN) were established for this study.Antigenicity of serotype 23F pneumococcal conjugates and their intermediates were analyzed by double immunodiffusion assay , inhibition ELISA and RN .The re-sults derived from three assays were comparatively analyzed to evaluate the changes of antigenicity during the preparing process of serotype 23F conjugate.Results Double immunodiffusion assay, inhibition ELISA and RN were all applicable to antigenicity analysis during the process of conjugate preparation .Inhibition ELISA could quantitatively detect a slight difference of polysaccharide antigenicity during the preparing process . Conclusion The antigenicity of polysaccharide during the preparing process of pneumococcal conjugates could be analytically monitored by using three immunological assays .This study provided evidence for suc-cessfully using immunological assays as the quality control means during the preparing process of pneumococ -cal conjugates .
