Raloxifene combined with calcium phosphate cement for repair of rabbit mandibular defects
10.3969/j.issn.2095-4344.2014.25.010
- VernacularTitle:磷酸钙人工骨联合雷洛昔芬修复兔下颌骨缺损
- Author:
Jian GUAN
;
Feng XU
- Publication Type:Journal Article
- Keywords:
biocompatible materials;
calcium phosphates;
raloxifene;
bone morphogenetic proteins
- From:
Chinese Journal of Tissue Engineering Research
2014;(25):3993-3997
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Raloxifene is the third generation of selective estrogen receptor modulators, which can decrease bone loss, increase bone mineral content, and reduce fracture risk. OBJECTIVE: To study the effects of raloxifene combined with self-setting calcium phosphate cement on the repair of rabbit mandibular defects. METHODS:Totaly 36 New Zealand white rabbits were selected to prepare 8 mm×4 mm×3 mm mandibular defect models, and then randomized equaly into experimental group (raloxifene, 7.5 mg/kg per day, combined with self-setting calcium phosphate cement), drug group (raloxifene, 7.5 mg/kg per day), artificial bone group (self-setting calcium phosphate cement). Rabbits were sacrificed 4, 8 and 12 weeks later, respectively, for measurement of bone morphogenetic protein 2 using immunohistochemistry method and transforming growth factor β using a laser scanning confocal microscope. RESULTS AND CONCLUSION:After 4 and 8 weeks, the expression of bone morphogenetic protein 2 was obviously higher in the experimental group than the drug and artificial bone groups; after 12 weeks, bone remodeling was basicaly complete in the experimental group, and the expression of bone morphogenetic protein 2 became lower than that in the other two groups. The expression of transforming growth factor β in the experimental group was gradualy increased and reached the peak at 8 weeks, while in the drug and artificial bone groups, the expression of transforming growth factor β exhibited an increasing trend within 4-12 weeks, which was close to the peak. These findings suggest that raloxifene can promote early expression of bone morphogenetic proteins and early calus formation as wel as accelerate the repair of bone defects with calcium phosphate cement.
