Molecular ecology of microbial communities in dental plaques of different caries-susceptible children
10.3969/j.issn.2095-4344.2014.25.019
- VernacularTitle:不同龋敏感儿童牙菌斑内微生物群落的分子生态学特点
- Author:
Lixia LIU
;
Lin CHEN
- Publication Type:Journal Article
- Keywords:
mouth;
dental plaque;
ecology
- From:
Chinese Journal of Tissue Engineering Research
2014;(25):4043-4050
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Children teeth filed with glass ionomer cement are stil susceptible with secondary caries, which is in close relationship with complex microbial community in dental plaque on the surface of glass ionomer cement. Traditional microbial methods are incapable of getting important information towards dental plaque microbes. OBJECTIVE:To analyze microbial community structure and numerical level of caries-induced microbes in dental plaque on the surface of glass ionomer cement for different caries-susceptible children. METHODS:Twenty-four children (age: 3-5 years) were divided into the caries-free, caries-positive, and caries-active children groups by the decayed, missing and filed index. With eight individuals in each group, their dental plaques were sampled for microbial community analysis. Denaturing gradient gel electrophoresis was employed to make clear the microbial community diversity and species identity in dental plaque of the caries-free, caries-positive, and caries-active children groups. Fluorescentin situ hybridization was used to investigate the numerical level of the caries-induced microbeStreptococcus spp. Quantitative PCR was carried out to analyze relative quantity ofStreptococcusmutans in total bacteria. RESULTS AND CONCLUSION:Compared with the caries-positive and caries-active children groups, microbial community diversity among samples was significantly higher in the caries-free group. Microbes abound in the caries-positive and caries-active groups might act important roles in the development of caries.Streptococcus spp. and Actinomyces spp. might be important caries-induced microbes in the caries-active group. The ratios ofStreptococcus spp. andStreptococcus mutans in total bacteria were significantly higher in the caries-free group than those in the caries-free and caries-positive groups. In summary, molecular ecology technologies can wel reflect caries-related complex microbial community in dental plaque.
