Homologx analxsis on Staphylococcus isolated from infectious wound-re-lated environmental specimens
10.3969/j.issn.1671-9638.2014.06.002
- VernacularTitle:感染创面相关环境标本分离葡萄球菌的同源性分析
- Author:
Ziyu QIAN
;
Binjun NI
- Publication Type:Journal Article
- Keywords:
Kex words] Staphylococcus;
Staphylococcusaureus;
coagulase negative Staphylococcus;
MRSA;
MRCNS;
multi-locus sequence analysis;
chromosome;
homology
- From:
Chinese Journal of Infection Control
2014;(6):327-331
- CountryChina
- Language:Chinese
-
Abstract:
Objective To monitor Staphylococcus infection in a hospital,and trace the source of Staphylococcus isolated from infectious wound-related hospital environment by molecular analysis. Methods By combination of fluorescence quanti-tative polymerase chain reaction (FQ-PCR)and culture method,environmental specimens related to 5 patients with wound infection were taken and performed multilocus sequence and MecA gene typing analysis. Results A total of 71 environmen-tal specimens were taken,Staphylococcus accounted for 36.62% (n= 26),88.46% (23/26 )of which were MecA+ methi-cillin-resistant strains. 77.78% (7/9)of Staphylococcus aureus (S. aureus)and 95.00% (19/20)of coagulase negative Staphylococcus (CNS)were methicillin-resistant strains. Multilocus sequence analysis revealed that ST239 (n= 6)was the most common sequence type in S. aureus;Staphylococcal cassette chromosome mec (SCCmec)analysis showed that the ma-jor type of S. aureus was typeⅢ,and CNS were typeⅢandⅣ. Conclusion Staphylococcus is common in healthcare-associated infection,and most Staphylococcus are multidrug-resistant,continuous monitor on drug-resistant Staph-ylococcus is necessary,and risk of Staphylococcus variant to medical institutes need to be paid attention.
