Effect and mechanism of Prunella vulgaris sulfated polysaccharide on angiogenesis in hepatocellular carcinoma
10.3969/j.issn.1000-8179.20140672
- VernacularTitle:夏枯草硫酸多糖对肝癌血管生成的作用及机制研究
- Author:
Yanan WANG
;
Rui CAO
;
Cong ZHU
;
Xiongzhi WU
- Publication Type:Journal Article
- Keywords:
prunella vulgaris sulfated polysaccharide;
Lentinan;
angiogenesis;
hepatocellular carcinoma;
microvessel density;
bFGF
- From:
Chinese Journal of Clinical Oncology
2014;(12):758-761
- CountryChina
- Language:Chinese
-
Abstract:
Objective:A study was conducted to investigate the effect of Prunella vulgaris sulfated polysaccharide (PVSP) on the expression of angiogenic growth factors (bFGF, VEGF, and IL-8) and angiogenesis in hepatocellular carcinoma. Methods:ELISA as-say was used to observe the effects of PVSP and the negative control drug Lentinan (LNT, non-sulfate radical drug) on secretions of the angiogenic growth factors, namely, bFGF, VEGF, and IL-8, in HepG2 cells in vitro. In an in vivo experiment, the microvessel density in hepatocellular carcinoma tissue sections treated with PVSP and LNT was calculated, analyzed, and compared with the microvessel den-sity in the phosphate-buffered saline (PBS) control. Results:Compared with the PBS control group, PVSP at 200μg/mL inhibited bF-GF secretion (P<0.01), whereas LNT failed to affect bFGF secretion. Neither PVSP nor LNT affected the secretions of VEGF and IL-8. In vivo results showed that PVSP at 200 mg/kg reduced the microvessel density in tumor tissue sections (P=0.03), whereas LNT at 10 mg/kg failed to affect microvessel density. Conclusion:Inhibition of bFGF secretion is a probable mechanism underlying the preven-tive effect of PVSP on hepatocellular carcinoma angiogenesis.
