The study of differences between eye acupuncture therapy and body acupuncture therapy on tumor necrosis factor-αexpression in rats with acute cerebral ischemia reperfusion
- VernacularTitle:眼针与体针对急性脑缺血再灌注损伤大鼠脑组织TNF-α表达的差异研究
- Author:
Huan JING
;
Dan YU
;
Yuan GAO
;
Zhe WANG
;
Ying WANG
;
Qian PAN
;
Hongquan GUAN
- Publication Type:Journal Article
- Keywords:
acute ischemia-reperfusion injury;
tumor necrosis factor-α;
eye acupuncture therapy;
body acupuncture therapy
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;(1):22-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of eye acupuncture and body acupuncture on tumor necrosis factor-α(TNF-α) expression in the cerebral cortex of rats after ischemia-reperfusion injury,in order to investigate the differences in therapeutic function between eye acupuncture and body acupuncture against acute cerebral ischemia-reperfusion injury. Method 48 Rats established by suture method were randomly divided into control group,sham operation group,model group,eye-acupuncture in point area group,eye-acupuncture outside point area group and body acupuncture group. After reperfusion 24 h,the neurophysical behaviours were accessed by ZeaLonga neurophysical impairment marks;the levels of plasma TNF-αwere determined by ELISA method;the expression of ischemic cerebral cortex TNF-αmRNA was measured by RT-PCR method;the expression of ischemic cerebral cortex TNF-αprotein was detected by western blot. Results After reperfusion 24 h,compared with control group,neurologic impairment marks of eye-acupuncture therapy in point area group and body acupuncture group both decreased obviously (P<0.01),however there were no significant differences between the eye-acupuncture in point area group and body acupuncture group;the levels of plasma TNF-αin rat cerebral cortex after the eye acupuncture therapy and body acupuncture therapy were obviously decreased (P<0.01),however there were no significant differences between the eye-acupuncture in point area group and body acupuncture group; the expressions of TNF-α mRNA and protein in rat cerebral cortex after the eye acupuncture therapy and body acupuncture therapy were also obviously down-regulated (P<0.01),however there were no significant differences between the eye-acupuncture in point area group and body acupuncture group.Conclusion The eye and body acupuncture therapy show the same effects on treating cerebral ischemia reperfusion. The mechanisms of these two therapies may be related to up regulating TNF-αexpression in rat cerebral cortex with ischemia-reperfusion injury.
