RGD and cell penetrating peptides co-modiifed paclitaxel loaded liposome inhibit the A549 cells
- VernacularTitle:RGD与细胞穿膜肽共修饰脂质体靶向抑制肺癌A549细胞的研究
- Author:
Weihua ZHANG
;
Min YE
;
Rongwei ZHOU
;
Jian SUN
- Publication Type:Journal Article
- Keywords:
integrin receptor;
cell penetrating peptides;
liposomes;
lung cancer
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;(1):46-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare RGD and TAT co-modified paclitaxel loaded liposome(RGD/TAT-LP-PTX)for A 549 cells targeting.Method The co-modified liposome was prepared by film-ultrasonic method. The appearance,particle size,Zeta potential were evaluated. The cellular uptake by A 549 cells in vitro was used to evaluate the targeting efficiency. The anti-proliferation efficiency of RGD/TAT-LP-PTX was evaluated by MTT assay. Results The particle diameter of the co-modified liposome was (118.5±11.4) nm with the Zeta potential of (21.58±2.42 )mV. The entrapment efficiency of PTX was 86.5%. The result demonstrated that the co-modified liposome uptaken by A 549 were 2.1, 2.8 times higher than that of TAT-LP and RGD-LP, respectively. The RGD/TAT-LP-PTX shows the highest anti-proliferation efficiency. Conclusion The co-modified liposome might serve as a promising tumor delivery system of antitumor drugs.
