The study of RGD modiifed paclitaxel and curcumin loaded liposome for the treatment of ovarian carcinoma in vitro and in vivo
- VernacularTitle:整合素受体介导共载紫杉醇和姜黄素脂质体的构建及其抗肿瘤研究
- Author:
Xiaowen ZHANG
;
Jing WU
- Publication Type:Journal Article
- Keywords:
integrin receptor;
paclitaxel;
curcumin;
liposome
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;(1):62-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare RGD conjugated paclitaxel and curcumin loaded liposome and evaluate their properties and effect on the treatment of ovarian carcinoma in vitro and in vivo . Method The liposomes were prepared by thin film hydration method. The particle size,Zeta potential and entrapment efficiency were evaluated. The efficiency of cellular uptake on A 2780 cells in vitrowas evaluated. Flowcytometry were used to observe the apoptosis morphous.A 2780 cells werexenografted in athymic mice to establish the animal model, which were used to evaluate the effect of anti-cancer. Results The particle diameter of the RGDLP-PTX/Cu was (94.8±11.2) nm with the Zeta potential of (-3±2.45) mV. The entrapment efficiency of PTX and Gen were 81.8%and 84.6%respectively. The result demonstrated that RGDLP uptaken by A 2780 were 3.6 times higher than that of LP. The apoptosis assay、the inhibition of the inhibition of tumor in vivoconfirmed strong inhibitory effect ofRGDLP-PTX/Cu. Conclusion RGDLP-PTX/Cu were easy to prepare and it is a potential delivery system for the treatment of ovarian carcinoma.
