Studies on the optimization of extraction method and content of different parts in XinJiang Bergenia crassifolia (L.)
- VernacularTitle:新疆厚叶岩白菜素提取方法优化及不同部位含量的研究
- Author:
Honghong WU
;
Lan PAN
;
Xiaoguang JIA
;
Minghui SHI
;
Mani KULIXIAXI
;
Xinyue JIA
- Publication Type:Journal Article
- Keywords:
bergenia crassifolia pigment;
alcohol extraction process;
high performance liquid chromatography
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;(1):74-75
- CountryChina
- Language:Chinese
-
Abstract:
Objective To extraction method optimization and the content of the different parts of Xinjiang Bergenia crassifolia (L.), provide the basis for efficient extraction of Bergenia crassifolia pigment. Method Using the orthogonal experiment, the alcohol extraction process of ethanol concentration, dosage of ethanol, extraction time, extraction times, as well as to Bergenia crassifolia pigment content of the different part. Results Of optimization, the best ethanol extract of xinjiang Bergenia crassifolia pigment process conditions is 8 times the amount of 50%ethanol, extracting 3 times, each time 0.5 h. In Bergenia crassifolia pigment content of different parts exists obvious difference. Bergenia crassifolia pigment content of Taproot and fibrous root is higher, at 8.65%to 9.58%, while Bergenia crassifolia pigment content of the leaves is relatively low, at just 0.15%. Bergenia crassifolia pigment content of leaves significantly higher than the old leaves. Conclusion This experimental study on efficient extraction of xinjiang Bergenia crassifolia pigment to provide strong technical support and theoretical basis.
