Preparation of pingyangmycin water-soluble wax stick and the content determination
- VernacularTitle:盐酸平阳霉素水溶蜡棒的研制及含量测定
- Author:
Juan LIU
;
Jun YAN
;
Fengshan WANG
- Publication Type:Journal Article
- Keywords:
pingyangmycin;
water-soluble wax stick;
preparation;
high performance liquid chromatography;
content determination
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;(1):82-84
- CountryChina
- Language:Chinese
-
Abstract:
Objiective To prepare pingyangmycin water-soluble wax stick and to establish its content determination method. Method The formula was optimized by orthogonal experiment and the content of pingyangmycin in wax stick was determined by high performance liquid chromatography (HPLC). Results The formula of pingyangmycin wax stick matrix was optimized as 1 g of alcohol ethoxylate and 1 g of S 40. Pingyangmycin was added when the temperature of the matrix raised to 70℃and stirred for 20 min. The linear range of pingyangmycin determined by HPLC was 34.4~172μg/mL,the regression equations was y=8298.9 x-34996(r=0.9999),and the average recovery of pingyangmycin was 102.67%(n=9). Conclusion The Preparing procedure of pingyangmycin water-soluble wax sticks is simple and stable. The HPLC method for determining the content of pingyangmycin in water-soluble wax sticks is simple, fast and accurate.
