Effects of MiR-194 on the metastasis of human osteosarcoma cell line U2-OS by recombinant lentivirus vector
10.3969/j.issn.1000-8179.20140226
- VernacularTitle:miR-194慢病毒表达质粒的构建及对人骨肉瘤细胞系U2-OS转移的相关研究
- Author:
Kang HAN
;
Tingbao ZHAO
;
Na BIAN
;
Chengkui CAI
;
Shiju YAN
;
Xin WANG
;
Qiong MA
;
Hao SHA
;
Chuan DONG
;
Tongtao YANG
;
Yong ZHOU
;
Baoan MA
- Publication Type:Journal Article
- Keywords:
osteosarcoma;
microRNA-194;
lentivirus;
U2-OS cell line;
metastasis
- From:
Chinese Journal of Clinical Oncology
2014;(12):753-757
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aimed to construct a lentiviral expression vector for microRNA-194 and investigate its effect on the metastasis of human osteosarcoma cell line U2-OS. Methods:Pri-and mature miR-194 amplified by PCR were inserted into the plenty-GFP vector and identified by restriction endonuclease digestion and nucleotide sequencing. The osteosarcoma cell line U2-OS was transfected with the lentivirus. Then, the stable transfected cells were used in Transwell and wound healing assay. Results:Restric-tion analysis and sequencing showed that the recombinant lentiviral expression vector was constructed correctly. The titers of obtained overexpression and suppression expression recombinant lentivirus were 1.5*108 and 4*108 TU/ml. Cell metastasis ability was signifi-cantly different in different experimental groups (P<0.01). Conclusion:The lentiviral expression vector for microRNA-194 was suc-cessfully constructed. MicroRNA-194 could influence the metastasis of the osteosarcoma cell line U2-OS;thus, it could be further ex-plored as a potential target in osteosarcoma therapy.
