Effect of Buzhong Yiqi Decoction on the expression of Caspase-3 protein of A549 tumor-bearing mice’spleen
- VernacularTitle:补中益气汤对A549荷瘤小鼠脾脏上Caspase-3蛋白表达影响研究
- Author:
Yu WANG
;
Chunying LIU
- Publication Type:Journal Article
- Keywords:
A549;
Caspase-3;
Buzhong Yiqi Decoction;
spleen
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;(1):19-21
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyses the effect of Buzhong Yiqi Decoction on the expression of Caspase-3 in human lung adenocarcinoma cells (A 549) tumor-bearing mice’spleen. Method 24 BALB/c mice were randomly divided into the control group(given saline),low-dose and high-dose Buzhong Yiqi Decoction groups,cisplatin(DDP)group;Buzhong Yiqi Decoction was administered twice daily for 10 days consecutively and DDP was administered once daily for 3 days consecutively, after 15 days was the spleens of all tumor-bearing mice were obtained.The expression of Caspase-3 protein and Caspase-3 mRNA in every group were detected by immunohistochemical method seperately and polymerase chain reaction method seperately. Results Compared with control group,the result of immunohistochemistry showed that the expression of the Caspase-3 protein in Buzhong Yiqi Decoction low-dose group and high-dose group were both ssignificantly increased (P<0.05). The differences hetween DDP group and control group was not significant, but the differences between DDP sroup and Buzhong Yigi Decoction Low-dose sroup and hishdoe group were significant (P<0.05, P<0.01).The result of RFPCR was accordance with the result of immunohisto chemistry. Conclusion Buzhong Yiqi Decoction can increase the expression of Caspase-3 protein in A549 ctumor-bearing mice’spleen,and promote the Caspase-3’s inducing effect on tumor cell apoptosis.
