Protective Effect of Tetramethylpyrazine on Caffeine-induced PC12 Cell Injury

VernacularTitle:川芎嗪对咖啡因引起的PC12细胞损伤的保护作用
Author: Jia WANG ; Feng GAO ; Chunbing ZHANG
Publication Type:Journal Article
Keywords: Tetramethylpyrazine; PC12 cells; Injury,cerebral ischemia-reperfusion
From: Herald of Medicine 2014;(6):695-698
CountryChina
Language:Chinese
Abstract: Objective To analyze whether tetramethylpyrazine could protect PC12 cells from injuries induced by caffeine,and to explore the mechanism of tetramethyipyrazine in the treatment of cerebral ischemia-reperfusion injury. Methods Caffeine was added to induce apoptosis of PC12 cells. Cytotoxicity was detected by CCK-8 assay. The electric potential of mitochondrial membrane was determined by flow cytometry. HMGB1 was detected by Western blotting. Oxidative stress was detected by ELISA. We observed toxicity of caffeine and the protective effects of tetramethylpyrazine. Results After the pre-treatment,tetramethylpyrazine significantly improved PC12 cell survival. Mitochondrial membrane potential was increased,the expression of HMGB1 decreased,SOD increased,LDH and MDA decreased,and GSH elevated. Conclusion Tetramethylpyrazine exerts a significant protective effect on PC12 cell injury caused by caffeine. The protective effect may be related to inhibition of apoptosis and regulation of the expression level of mediators involved in inflammation and oxidative stress.