Inhibitory effect of melatonin on murine foregastric carcinoma cells via membrane receptors MT2
10.3969/j.issn.0529-1356.2014.03.009
- VernacularTitle:M T2受体介导褪黑素对小鼠前胃癌细胞的抑制作用
- Author:
Li XU
;
Qingdong JIN
;
Xi GONG
;
Hui LIU
;
Ruixiang ZHOU
- Publication Type:Journal Article
- Keywords:
Melatonin;
Murine foregastic carcinoma cell;
Melatonin membrane receptor MT 2;
Akt;
ERK1/2;
Real-time PCR;
Mouse
- From:
Acta Anatomica Sinica
2014;(3):344-349
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of melatonin on the proliferation activity of murine foregastic carcinomac ( MFC) cells via melatonin membrane receptors MT 2 and its relationship with the signaling pathways of mitogen-activated protein kinases ( MAPKs), phosphatidylinositol 3-kinase ( PI3K)-Akt.Methods Using siRNA technology to silence MT2 expression, we examined the ability of melatonin to inhibit the proliferation activity of MFC cells and its influence on the phosphorylation of ERK 1/2 and Akt.Results We found two interesting effects of SiRNA-mediated silencing of MT2 expression.Firstly, it significantly antagonized the inhibitory effect of melatonin on the proliferation activity of MFC cells .Secondly , it partially blocked the inhibitory effect of melatonin on the phosphorylation of ERK 1/2 and Akt.Conclusion Our results suggest that melatonin can inhibit the phosphorylation of ERK 1/2 and Akt via MT2 receptors , thereby inhibiting the proliferation of gastric cancer cells .
