Expression of tumor stem cell markers in the tumor sphere from esophageal squamous cells carcinoma Eca109
10.3969/j.issn.0529-1356.2014.03.010
- VernacularTitle:多种肿瘤干细胞标志物在食管鳞癌 Eca109细胞球细胞中的表达
- Author:
Yanyan HUANG
;
Xuelian PEI
;
Yang LIU
;
Qiuxia LI
;
Yuting WANG
;
Xiaoling MU
- Publication Type:Journal Article
- Keywords:
P75NTR;
Oct-4;
Sox-2;
Lin28;
Nanog;
Cancer stem cell;
Esophageal squamous cell carcinoma;
Tumor sphere formation
- From:
Acta Anatomica Sinica
2014;(3):350-353
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of tumor stem cell markers P 75NTR,Oct-4,Sox-2,Lin28 and Nanog in the tumor sphere from esophageal squamous cells carcinoma Eca 109 and identify the esophageal squamous cell cancer stem cell marker .Methods The serum-free culture method was used for generating tumor spheres: proliferation was observed in enrichment culture tumor spheres .Small tumor spheres were obtained after 5 days culture and big and round tumor spheres appeared after 14 days culture which were collected for experiments and passaged .The expression and location of P75NTR,Oct-4,Sox-2,Lin28, and Nanog were detected by immunofluorescence cytochemistry .Results The expressions of P75NTR,Oct-4 and Lin28 were positive in the center of tumor spheres and some on cytoplasm and other in nuclei of Eca109 monolayer cells.However, Oct-4 fluorescence intensity was weaker than P75NTR.The expressions of Sox-2 and Nanog were positive in cytoplasm of tumor spheres and Eca 109 monolayer cells .Conclusion The cells expressing P75NTR, Oct-4, and Lin28 in the center of the tumor sphere may be esophageal cancer stem cells .
