Effects of Cdc20 mutation on growth of mouse embryonic fibroblast
10.3969/j.issn.0529-1356.2014.03.016
- VernacularTitle:Cdc20基因突变对 APCmin／＋小鼠胚胎成纤维细胞生长的影响
- Author:
Juling FENG
;
Lei ZHAO
;
Juan XIE
;
Mingshu MO
;
Qingjun GUI
;
Yong YOU
;
Hui ZHONG
;
Lisheng WANG
- Publication Type:Journal Article
- Keywords:
Cdc20;
APCmin/+;
Embryonic fibroblast;
Foci formation assay;
Karyotype analysis;
Chromosome;
Instability;
Mouse
- From:
Acta Anatomica Sinica
2014;(3):383-387
- CountryChina
- Language:Chinese
-
Abstract:
Objective Investigation of biological characteristics of Cdc 20AAA/+APCmin/+ mouse embryonic fibroblast(MEFs) indicate the effect of Cdc20AAA/+on growth of mouse embryonic fibroblast and the possible mechanism . Methods MEFs of Cdc20AAA/+APCmin/+, Cdc20AAA/+, APCmin/+ and WT genotype were harvested from embryos for analysis.The growth characteristics of Cdc20AAA/+APCmin/+, Cdc20AAA/+,APCmin/+and WT mouse embryonic fibroblast were analyzed through growth curve analysis and foci formation assay .Separation of sister chromatid and the presence of aneuploid were detected by karyotype analysis .Results Cell proliferation assays showed that Cdc 20AAA/+APCmin/+cells grew at an accelerated rate compared with APC min/+MEFs(P<0.01).Foci formation assay showed that the clone forming ability was significantly increased .Cdc20AAA/+APCmin/+MEFs showed a significant increase in the frequency of aneuploid compared with WT MEFs , which had a karyotype of 38 and contained prematurely separated sister chromatids .Conclusion Cdc20 carrying a null allele (Cdc20AAA/+) may accelerate the growth and proliferation of APC min/+MEFs and present the growth characteristics of the tumor cells .The possible mechanism may be associated with chromosome instability .
