Effect of stromal cell derived factor-1αand interleukin-1βon inducing vascular endothelial cells expressing lymphatic phenotype
10.3969/j.issn.0529-1356.2014.03.017
- VernacularTitle:基质细胞衍生因子-1α和白细胞介素-1β诱导淋巴管内皮表型的作用
- Author:
Ning SUO
;
Xueying WANG
;
Chunlin YANG
;
Hui ZHOU
;
Fei LI
;
Zongpu ZHANG
;
Fangzhu WAN
;
Hua TIAN
- Publication Type:Journal Article
- Keywords:
Stromal cell-derived factor-1α;
Interleukin-1β;
Prospero ho-meobox protein-1;
Vascular endothelial cell;
Real-time PCR
- From:
Acta Anatomica Sinica
2014;(3):388-392
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of stromal cell-derived factor-1α( SDF-1α) and interleukin ( IL-1β) on inducing vascular endothelial cells to express lymphatic phenotype .Methods The CRL-1730 cell line was cultured and treated with SDF-1αor IL-1β.The expression of endothelial cell markers and lymphatic endothelial cell markers were investigated with Real-time PCR, Western blotting and immunocytochemistry .Results In CRL-1730 cell line, endothelial cell markers such as voln willebrand factor ( vWF ) , VE-cadherin , vascular endothelial growth factor receptor(VEGFR)2, were dose dependently down-regulated after SDF-1αstimulation, while lymphatic phenotypes such as Prox-1, podoplanin and lymphatic vessel endothelial hyaluronan receptor-1(LYVE-1), were dose-dependently up-regulated after SDF-1αstimulation.The changes of vWF, VEGFR2 and podoplanin, Prox-1, LYVE-1 expression after IL-1βstimulation was similar to that after SDF-1αwhile expression of VE-cadherin changed slightly .Conclusion SDF-1αand IL-1βare able to induce vascular endothelial cell expressing lymphatic phenotype .
