Effect of Pirfenidone on Activity of Hepatic Cytochrome P450 in Rats
10.3870/yydb.2014.06.008
- VernacularTitle:吡非尼酮对大鼠肝细胞色素P450酶活性的影响
- Author:
Ji WU
;
Hui CHEN
;
Jianhong WU
;
Shunchang ZHOU
;
Fandian ZENG
- Publication Type:Journal Article
- Keywords:
Pirfenidone;
Cytochrome P450;
Enzyme activity
- From:
Herald of Medicine
2014;(6):723-726
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of pirfenidone on total enzyme and isoenzyme of liver microsomal cytochrome P450 in rats. Methods The activites of liver microsomal dimethyl nitrosamine N-demethylase( NDMA )and erythromycin demethylase( ERD ) were determined. Fifty-six SD rats were randomly divided into six groups,in which they received CMC,dexamethasone 100 mg·kg-1 ,ketoconazole 40 mg·kg-1 ,pirfenidone 25,50,100 mg·kg-1 ,respectively. After administration for 6 and 12 days,livers were prepared liver microsome,the concentration of proteinum in microsome and shade selection to plasma samples were determined by spectrophotometer. Results After administration of pirfenidone for 6 days, cytochrome P450 was significantly increased in 50 and 100 mg·kg-1 pirfenidone groups,as compared with solvent control group (1%sodium carboxymethylcellulose)(P﹤0. 01). After administration of pirfenidone for 6 and 12 days,NDMA of liver microsome was not changed significantly(P﹥0. 05). ERD of liver microsome was significantly increased in 100 mg·kg-1 pirfenidone group after administration for 12 days(P﹤0. 01). Conclusion Pirfenidone can induce P450 and ERD activities in a dose-and time-dependent manner.
