Collapsin response mediator protein 5 accelerates neurite outgrowth in hippocampal neurons
10.3969/j.issn.0529-1356.2014.03.001
- VernacularTitle:坍塌反应调节蛋白5促进海马神经元突起生长
- Author:
Keen CHEN
;
Yuanyuan WANG
;
Jifeng ZHANG
;
Bin LI
;
Guoqing GUO
- Publication Type:Journal Article
- Keywords:
Collapsin response mediator protein 5;
Neuron;
Neurite;
Outgrowth;
Gene transfection;
Eukaryotic expression;
Western blotting;
Rat
- From:
Acta Anatomica Sinica
2014;(3):297-303
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate function of collapsin response mediator protein 5 ( CRMP5 ) on neurite outgrowth.Methods The CRMP5 eukaryotic expression vector was constructed and transfected into hippocampal neurons . The gene transfection, Real-time quantitative PCR and Western blotting were used to detect expression of CRMP 5 protein. The lapse-time imaging and neurite extraction were utilized to show neurite outgrowth and differentiation and 3 double-pored were performed, compared with the vector without CRMP5 gene.Results It was successful to construct the CRMP5 eukaryotic expression vector with an EGFP tag .The lipofectamine effectually transfected CRMP 5 into cultured neurons , and the CRMP5 protein was expressed successfully more than the control cells .CRMP5 protein was abundant in cell body , initiation and end of neurites .Overexpression of CRMP5 in neuronal cells significantly promoted outgrowth neurites , and led to the formation of longer neurites with more branches .Accompanying rapid outgrowth of neurites , branches from original neurites were contributed to form a network .The results of neurite length and extraction showed that neurons overexpressing CRMP5 were possessed more and longer neurites (P<0.01), compared with control cells .Conclusion The results suggest that CRMP5 accelerates not only axonal growth but also branching .
