Mortalin promotes ovarian cancer cell growth through MAPK-ERK signal pathway
10.3969/j.issn.0529-1356.2014.03.008
- VernacularTitle:上调分子伴侣 mortalin 经由 MAPK-ERK 信号转导通路促进卵巢癌细胞增殖
- Author:
Yingying HU
;
Yanyan HAN
;
Jiawei ZHAO
;
Ling YANG
;
Wen LIU
;
Ji ZUO
- Publication Type:Journal Article
- Keywords:
Mortalin;
Ovarian cancer;
Cell cycle;
MAPK-ERK signaling pathway;
Plasmid construction;
CCK-8;
Western blotting;
Human
- From:
Acta Anatomica Sinica
2014;(3):338-343
- CountryChina
- Language:Chinese
-
Abstract:
Objective By constructing mortalin stably expressing ovarian cancer cell lines in A 2780 and A2780/cis, we demonstrate the role of mortalin in the ovarian cancer cell growth .Methods CCK-8 assay was used to measure cell viability in the overexpression mortalin group compared with the control group .The flow cytometry analysis was used to understand the effect of upregulated mortalin on the ovarian cancer cell cycle .Western blotting was used to determine the expression and phosphorylation level of MAPK /ERK and JNK/SAPK signal pathways .Results The results showed that increased expression of mortalin could accelerate ovarian cancer cell proliferation and promote G 1 transition, leading to a faster restoration of normal distribution of cell cycle .We found that mortalin overexpression significantly activated p-Raf and p-ERK1/2, but not p-JNK.Conclusion The results demonstrate that mortalin effect on the ovarian cancer cell proliferation contributes to active the MAPK-ERK signaling pathway .
