Effects of 5-aza-2-deoxycyfidine on proliferation and activation of myofibroblast in arthrofibrosis of the knee
10.3969/j.issn.1006-5725.2014.10.004
- VernacularTitle:甲基转移酶抑制剂5-Aza-dC对膝关节纤维化中肌成纤维细胞活化增殖的影响
- Author:
Hao WANG
;
Kun WANG
- Publication Type:Journal Article
- Keywords:
5-aza-2-deoxycyfidine;
Arthrofibrosis of the knee;
Myofibroblast;
DNA methylation
- From:
The Journal of Practical Medicine
2014;(10):1526-1530
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of methyltransferase inhibitor 5-Aza-dC (5-aza-2’-deoxycytidine) on proliferation and activation of myofibroblasts in arthrofibrosis of the knee. Methods The model of arthrofibrosis of rat knee was firstly established.Myofibroblasts were isolated and cultured from the posterior capsule of rat knee. Cells were identified by immunofluorescence.Myofibroblasts were treated with 2 μmol/L 5-Aza-dC. The expression levels ofα-SMA、col1A1 mRNA and protein in myofibroblasts before and after the treatment with 5-Aza-dC were detected by RT-PCR and Western blotting,respectively.The proliferation rate of these myofibroblasts were detected by MTT method,and the cell cycle was detected by flow cytometry of DNA content in each phase. Results The extension of rat knee in arthrofibrosis model was significantly decreased. α-SMA protein,the representational protein of myofibroblast,was largely expressed in the isolated cells from model knees. The expression levels ofα-SMA、col1A1 mRNA and protein in myofibroblasts were decreased after the treatment with 5-Aza-dC and the growth of myofibroblasts was also slowed down. Conclusion The methyltransferase inhibitor 5-Aza-dC may become a potential therapeutic drug in the treatment of arthrofibrosis of the knee through inhibiting the proliferation of myofibroblasts.
