The influence of inactive frmd4a gene on the biological behavior of human tongue cancer cal-27 cell
10.3969/j.issn.1006-5725.2014.10.008
- VernacularTitle:特异性沉默FRMD4A基因对人舌癌CAL-27细胞生物学行为的影响
- Author:
Xianghuai ZHENG
;
Jianjiang ZHAO
;
Bo JIA
;
Jie PAN
;
Jun CHEN
;
Xiaoling QIU
;
Jiusong HAN
;
Hongxing CHU
- Publication Type:Journal Article
- Keywords:
Tongue neoplasms;
Gene silencing;
FRMD4A;
Cell proliferation;
Cell cycle;
Wound healing
- From:
The Journal of Practical Medicine
2014;(10):1541-1544
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of inactive FRMD4A gene′s expression on the biological behavior of tongue cancer CAL-27cell. Methods FRMD4A-siRNA was transfered into CAL-27 cell by lipidosome, to the expression of FRMD4A-siRNA in CAL-27 cell after transfection was detect by qRT-PCR cell proliferation , was checked by CCK-8,the influence of inactive FRMD4A gene on cell cycle distribution of CAL-27 cell was assayed by flow cytometry. Results Compared with the control group, FRMD4A mRNA expression significantly reduced in FRMD4A-siRNA interfering group (94%) and the cell proliferation index decreased(P<0.05). The cell cycle arrested in G1 period (P<0.05). Conclusion FRMD4A-siRNA could effectively inhibit FRMD4A mRNA expression in tongue cancer CAL-27cell, impact the distribution of cell cycle, and reduce cell proliferation.
