Establishment of homogeneous chemiluminescent immunoassy for detecting serum cardiac troponin I
10.3969/j.issn.1006-5725.2014.10.043
- VernacularTitle:血清肌钙蛋白-I均相化学发光免疫检测方法的建立
- Author:
Yuqin LI
;
Wenyan NIU
;
Lina ZHU
;
Yujie ZHOU
;
Ping XIANG
;
Liying HOU
;
Kegang JIA
;
Huiqiang LI
- Publication Type:Journal Article
- Keywords:
Cardiac troponin I;
Homogeneous immunoassay;
Detection method;
Light induced chemilum-inescence
- From:
The Journal of Practical Medicine
2014;(10):1640-1643
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish homogeneous immunoassay for detecting serum cardiac troponin I (cTnI) by using light induced chemiluminescent immunoassay (LiCA). Methods Polyclonal antibodies of cTnI were coated on the receptor particles, monoclonal antibodies of cTnI were biotinylated, and the donor particles were coated with streptavidin, all of which were composed of LiCA reagents. The optimal test conditions and analytical performance of the detection method were studied. Results The method was rapid, sensitive, and detection time was 17.5 min.The analytical sensitivity was 0.045 ng/mL and the functional sensitivity was 0.053 ng/mL.The recovery rate was 104.96%-108.21%;The within-run and the between-run coefficients of variation were 3.88%-5.53%and 7.60%-8.75%, respectively. The interference rates for the endogenous substances were less than 10%. The reference value of cTnI was less than 1.05 ng/mL;Results of cTnI LiCA correlated well with direct chemiluminescence detection (r2 =0.979). Conclusions This approach can be used for the quantitative detection of serum cTnI, and it is homogeneous and is free of clean separation. It provides a convenient, highly sensitive detection platform for clinical practice.
