Protective effect of schisandrin B against HK-2 apoptosis induced by Cisplatin
10.3969/j.issn.1001-1978.2014.07.017
- VernacularTitle:五味子乙素抗顺铂所致HK-2凋亡及其机制的研究
- Author:
Yanhong WANG
;
Xiang XU
- Publication Type:Journal Article
- Keywords:
schisandrin-B;
Cisplatin;
nephrotoxicity;
HK-2;
apoptosis;
oxidative stress
- From:
Chinese Pharmacological Bulletin
2014;(7):961-964
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the protective role of schisandrin B (SchB)against HK-2 damage induced by Cisplatin and the probable mechanism.Methods Cultivated HK-2 was interacted by Cisplatin and SchB with different concentrations.Invert microscope showed cellular form change and Flow Cyto Meter told apopto-sis.Combined with test result on the change of L-DH, MDA and LPO,probable protective effect of SchB on HK-2 was studied and analyzed.Result Cisplatin in-hibited cells′growth and induced apoptosis.20 μmol · L-1 SchB significantly reduced apoptosis of Cispla-tin-induced HK-2,and offered the best protection. SchB could reduce MDA,LPO,LDH significantly in-creased by cisplatin.Conclusion SchB can be an ef-fective inhibition agent of Cisplatin-induced renal tubu-lar epithelial cell apoptosis (HK-2 ).The mechanism can be the inhibition of Cisplatin-induced oxidative stress by antioxidant effect.
