Study on the role of COX-2 in BMP9 induced osteogenic differentiation in mesenchymal stem cells
10.3969/j.issn.1001-1978.2014.07.026
- VernacularTitle:环氧酶-2在骨形态蛋白9诱导间充质干细胞骨向分化中的作用研究
- Author:
Jun HUANG
;
Yingzi LIU
;
Shuangxue YUAN
;
Qiuxiang WU
;
Dongxu WANG
;
Qixin ZHOU
;
Baicheng HE
- Publication Type:Journal Article
- Keywords:
COX-2;
BMP9;
mesenchymal stem cells;
osteogenic differentiation;
BMPs/Smads signaling;
phosphorylation
- From:
Chinese Pharmacological Bulletin
2014;(7):1006-1011
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the role of COX-2 in BMP9 induced osteogenic differentiation,and the pos-sible mechanism underlying this function of COX-2. Methods We introduced real-time PCR, Western blot, and immunocytochemical staing to detect the effect of BMP9 on COX-2 expression.We employed chemiluminescence technique to assay ALP activities, RT-PCR to detect the expression of Smad6 and Smad7 , and Western blot to measure the expression of Runx2, Dlx-5,total Smad1/5/8,and phosphorylated Smad1/5/8.Finally,BMPR-Smad luciferase reporter assay was applied to measure the activation of BMPs/Smads signaling.Results BMP9 could induce the expression of COX-2 in C3H10T1/2 cells.Either inhibiting enzy-matic activity of COX-2 or knockdown of the expression of COX-2 reduced the BMP9 induced ALP activities in C3H10T1/2 cells,and COX-2 knockdown also inhibited the ectopic bone formation induced by BMP9 in C3H10T1/2 cells.Moreover,COX-2 knockdown inhibi-ted BMPR-Smad reporter activities and the phosphoryl-ation of Smad1/5/8,so did the expression of Smad6 and Smad7 .Conclusion COX-2 may play an impor-tant role in BMP9 induced osteogenic differentiation in MSCs by regulating the BMPs/Smads signaling trans-duction.
