Determination of Four Components in Fresh Houttuynia cordata from Different Harvest Time with Gas Chromatography-Mass Spectrometry
10.11842/wst.2014.06.032
- VernacularTitle:GC-MS法测定不同采收期鲜品鱼腥草挥发油中4种成分的含量
- Author:
Gang HE
;
Guangming QING
;
Min LI
;
Xingjun YANG
;
Yuanhong LUO
;
Min REN
- Publication Type:Journal Article
- Keywords:
Houttuynia cordata;
GC/MS;
4-terpineol;
α-terpineol;
bornyl acetate;
methyl-n-nonylketone
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(6):1391-1395
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to establish a method for assay of 4-terpineol, α-terpineol, bornyl acetate and methyl-n-nonylketone in Houttuynia cordata with gas chromatography-mass spectrometry (GS/MS) for the first time in order to provide experimental evidences for its quality control. Undecylen was analyzed quantitatively. GC/MS method was used in the content determination of 4 kinds of components in Houttuynia cordata. GC conditions were that the temperature was firstly increased to 70oC for 5 min, and then 5oC·min-1 to 140oC for 5 min. Then, the temperature was increased by 10oC·min-1 to 250oC for 10 min. The temperature of the injection port was 230oC. The carrier gas was high purity helium. The flow rate was 1.0 mL·min-1. The split ratio was 10:1. The sample quantity was 1 μl. MS conditions were that the detector was MS, EI was 70ev, the temperature of four poles was 150oC, the temperature of ion source was 230oC. The results showed that there was good linearity of four components, which were 4-terpineol, α-terpineol, bornyl acetate and methyl-n-nonylketone obtained within the ranges of 6.492~129.84μg·min-1, 1.097~21.944 μg·min-1, 12.128~242.56 μg·min-1, and 84.76~169.52 μg·min-1, respectively. The average recoveries of the four components were 81.2%, 80.6%, 88.3%, and 84.6%, respectively. The RSDs were 1.5%, 2.3%, 1.1%, and 0.7%, respectively. It was concluded that the method was simple, sensitive, and easy to operate. The simultaneous measurement of multiple indexes on the component for quality evaluation of Houttuynia cordata can effectively reflect the quality of Houttuynia cordata. It can also be used in the quality assessment of fresh Houttuynia c ordate .
