Study on Tetrahydropulmatine Preparation with Fibraurea recisa Pierre
10.11842/wst.2014.06.038
- VernacularTitle:用黄藤制备延胡索乙素的方法研究
- Author:
Guang LIU
;
Guihua GENG
;
Na QI
- Publication Type:Journal Article
- Keywords:
Fibrauretine;
tetrahydropulmatine;
extract;
isolate
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(6):1422-1426
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to extract and separate fibrauretine from Chinese herbal medicine Fibraurea recisa Pierre. and to prepare tetrahydropulmatine with fibrauretine. Extraction method of fibrauretine were optimized by the L9(34) orthogonal arrays as experimental designs. Then, elution chromatography method was studied with the macroporous adsorption resin chromatography technology. At last, tetrahydropulmatine was prepared by the reaction of sodium borohydride hydrogenating fibrauretine. The results showed that the content and yield of obtained fibrauretine were 36.40% and 3.17%, respectively. The reflux exacted methods of fibrauretine were with 10 times amount (v/w) of 50%ethanol solution for 2 hours and 3 times. Then, HPD-300 macroporous resin was used to absorb fibrauretine, and purify water. The 20% and 60% ethanol solution was used for elution. The content and yield of obtained fibrauretine were 65.47% and 82.44%, respectively. And the content and yield of obtained tetrahydropulmatine were 98.31% and 72.04%, respectively. It was concluded that the method can obtain fibrauretine with relative high content and yield. Using this material can obtain tetrahydrofibrauretine with relative high content and yield.
