Influence of dihydroxy-stilbene compound Vam3 on ATP-induced inflammatory response in macrophages and the underlying mechanisms
10.3969/j.issn.1001-1978.2014.07.011
- VernacularTitle:二苯乙烯化合物Vam3对ATP诱导的小鼠腹腔巨噬细胞炎症反应的影响及机制研究
- Author:
Shuhua CAO
;
Chunsuo YAO
;
Qi HOU
- Publication Type:Journal Article
- Keywords:
Vam3;
macrophage;
ATP;
P2X7;
in-flammation;
Ca2+
- From:
Chinese Pharmacological Bulletin
2014;(7):931-936
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of Vam3 on ATP-induced inflammatory response in macrophages and the underlying mechanisms. Methods LPS primed mouse peritoneal macrophages were stimulated with ATP,and IL-1βlevel in supernatants was meas-ured by ELISA.Activity of caspase 1 was measured u-sing caspase 1 activity assay kit.Reactive oxygen spe-cies (ROS )level was detected with fluorescent probe DCFH-DA.MTT assay was used to detect cell prolifer-ation,and intracellular Ca2+concentration was meas-ured using laser scanning confocal microscope.Results Extracellular ATP led to increase in IL-1βrelease, caspase 1 activity and ROS production.It also led to rapid increase in intracellular Ca2+concentration and induced cell death.These effects were inhibited by Vam3 .Conclusion Vam3 inhibits ATP-induced in-flammatory response in macrophages,which may sug-gest the blocking effect of Vam3 on caspase 1 ~IL-1βinflammatory signaling pathway in macrophages.
