Inhibitory effect of lentivirus targeting interference Cx26 on proliferation and migration of human highly metastatic hepatocellular carcinoma HCCLM3 cells
10.3969/j.issn.1001-1978.2014.07.012
- VernacularTitle:慢病毒靶向干扰Cx26抑制人高转移性肝癌HCCLM3细胞增殖及迁移
- Author:
Guihui QIN
;
Junze CHEN
- Publication Type:Journal Article
- Keywords:
connexin 26;
gap junction;
hepatocellular carcinoma;
proliferation;
apoptosis;
migration;
lentiviral vector
- From:
Chinese Pharmacological Bulletin
2014;(7):937-941
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate effects of Cx26 on pro-liferation,apoptosis,migration of human highly meta-static hepatocellular carcinoma HCCLM3 cells.Meth-ods The HCCLM3 cells were infected with lentiviral vector targeting interference of Cx26 and the stable transfectants were selected by puromycin.The interfer-ence efficiency of Cx26 was detected by Real-time PCR and Western blot.Gap junction function was assessed by “parachute”dye-coupling assay. The effects of Cx26 interference on proliferation,apoptosis,migra-tion of HCCLM3 cells were determined by MTT assay, flow cytometry,transwell migration assay,respective-ly.Results The mRNA and protein expression of Cx26 in LV-Cx26 group was significantly lower than the LV-NC group and wide group (P<0.01),and GJ function in LV-Cx26 group also significantly reduced compared with LV-NC group and wild group (P <0.01 ).The Cx26 interference significantly inhibited the proliferation (P<0.01)),promoted the apoptosis (P <0.01 ),and decreased migration of HCCLM3 cells in vitro (P <0.01 ).Conclusion Lentiviral vector targeting interference Cx26 expression of HC-CLM3 cells can significantly reduce its GJ function,in-hibit the proliferation and migration,promote apopto-sis,and reduce its malignant properties.
