Chloride currents activated by cisplatin in poorly differentiated naso-pharyngeal carcinoma cells are not Ca2+-activated chloride currents
10.3969/j.issn.1000-4718.2014.06.002
- VernacularTitle:顺铂激活的低分化鼻咽癌细胞氯电流为非钙激活氯电流
- Author:
Xiaoya YANG
;
Mei LIU
;
Jiabao WU
;
Zhouyi LAI
;
Yuan WANG
;
Aihui FAN
;
Linyan ZHU
;
Jianwen MAO
;
Liwei WANG
;
Lixin CHEN
- Publication Type:Journal Article
- Keywords:
Cisplatin;
Nasopharyngeal neoplasms;
Chloride channels;
Calcium
- From:
Chinese Journal of Pathophysiology
2014;(6):968-974
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the type of chloride channel activated by cisplatin in poorly differentiated na -sopharyngeal carcinoma cells (CNE-2Z cells).METHODS:The technique of whole-cell patch-clamp was used to investi-gate the role of Ca 2+in the activation of cisplatin-activated chloride currents and to analyze the effect of hypertonic stress on these currents in CNE-2Z cells.RESULTS:Chloride currents were induced when the cells were exposed to the calcium -free cisplatin solution , showing the similar density to the currents induced by cisplatin with the presence of extracellular cal -cium.However , the latency and the peak time of cisplatin-activated currents in the absence of extracellular calcium were prolonged.The activation of cisplatin-activated chloride currents was insensitive to the depletion of intra-and extracellular calcium.Calcium channel antagonist nifedipine had no effect on the cisplatin -activated chloride currents , while hypertonic solution completely inhibited those currents .CONCLUSION:The cisplatin-activated chloride currents are independent on intra/extracellular calcium .The chloride channels activated by cisplatin are not calcium-activated chloride channels , but are probably volume-sensitive chloride channels .
