Effects of p21-activated protein kinase 2 down-regulation on proliferation and apoptosis of human breast cancer cells
10.3969/j.issn.1000-4718.2014.06.003
- VernacularTitle:下调 p21活化蛋白激酶2表达对人乳腺癌细胞增殖和凋亡的影响
- Author:
Xiang LI
;
Xiaoyan ZHANG
;
Jimin ZHAO
;
Kangdong LIU
;
Mingyao ZHAO
;
Ziming DONG
- Publication Type:Journal Article
- Keywords:
p21-activated protein kinase 2;
Breast neoplasms;
RNA interference;
Cell proliferation;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2014;(6):975-981
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the effect of p21-activated protein kinase 2 (PAK2) knockdown by RNA interfer-ence on the proliferation and apoptosis of human breast cancer cells .METHODS:The short hairpin RNA ( shRNA) targe-ting PAK2 gene was designed and used for packing lentivirus in 293T cells.Human breast cancer MCF-7 cells were infected by the virus particles and PAK2 knockdown stable cell line was established by puromycin selection .The knockdown effi-ciency was assessed by Western blotting .The proliferation ability of MCF-7 cells was evaluated by CellTiter 96 AQueous and anchorage-independent growth assays .The cell apoptosis induced by staurosporine was detected by flow cytometry . RESULTS:The protein level of PAK2 was significantly suppressed after silencing of PAK2 gene in MCF-7 cells ( P<0.01).Furthermore, knockdown of PAK2 caused remarkable inhibition of the cell proliferation and colony formation (P<0.01).Staurosporine induced more apoptosis in the PAK2 knockdown cells compared with the control cells (P<0.01). CONCLUSION:Knockdown of PAK2 inhibits the proliferation of MCF-7 cells and increases the sensitivity of chemothera-peutic drug-induced cell apoptosis , suggesting that PAK2 might be a new therapeutic target in breast cancer treatment .
