Survivin-2B induces apoptosis of human breast cancer cells
10.3969/j.issn.1000-4718.2014.06.004
- VernacularTitle:Survivin-2B诱导人乳腺癌细胞凋亡的初步研究
- Author:
Wenfeng ZHANG
;
Jun JIA
;
Fenglin WU
;
Han SHEN
;
Hongwei SHAO
;
Shulin HUANG
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Breast neoplasms;
Survivin-2B
- From:
Chinese Journal of Pathophysiology
2014;(6):982-987
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the role of survivin-2B in the process of tumor cell apoptosis .METHODS:The survivin-2B gene was cloned into pcDNA3.1 vector and the recombinant plasmid pcDNA3.1-survivin-2B was obtained.Hu-man breast cancer MCF7 cells were transfected with pcDNA3.1 and pcDNA3.1-survivin-2B using Lipofectamine 2000.The cell cycle was determined by propidium iodide staining , and the apoptosis was detected by annexin V/7-AAD staining 48 h after transfection.Meanwhile, tatal RNA was extrated and multiplex polymerase chain reaction based on GenomeLab GeXP Genetic Analysis System was performed to detect the expression of 21 tumor-related genes .RESULTS: Flow cytometry analysis indicated that over-expression of survivin-2B promoted the apoptosis and cell cycle arrest of MCF 7 cells.Compared with control group , totally 10 differential expressed genes were related to the over-expressed survivin-2B, among which 2 were up-regulated and 8 were down-regulated. The expression of aldehyde dehydrogenase 4 family member A1 (ALDH4A1) was 48%down-regulated, and the expression of protein regulator of cytokinesis 1 (PRC1) was 1.08 folds up-regulated.CONCLUSION:Survivin-2B induces the expression changes of some tumor-related genes, which results in the apoptosis and G 2/M arrest of MCF7 cells.
