Minocycline inhibits BV-2 cell activation by regulating P2X7 receptor

VernacularTitle:米诺环素调控P2X7受体抑制BV-2细胞活化的研究
Author: Shuqiong LIU ; Lianhong YANG ; Longyuan JIANG ; Jinhao YE
Publication Type:Journal Article
Keywords: Minocycline; Receptors,P2X7; Tumor necrosis factor α; Interleukin 1β
From: Chinese Journal of Pathophysiology 2014;(6):1066-1069
CountryChina
Language:Chinese
Abstract: AIM: To explore the role of P2X7 receptor in inhibition of lipopolysaccharide (LPS)-stimulated BV-2 cell activation by minocycline .METHODS:BV-2 cells were divided into 5 groups:control group, LPS group, LPS+0.1 μmol/L Mino group, LPS+1 μmol/L Mino group and LPS+10 μmol/L Mino group.The expression of P2X7 re-ceptor was determined by real-time PCR and Western blotting .The levels of TNF-αand IL-1βin the microglia culture su-pernatants were measured by ELISA .The morphological changes of the cells were also observed .RESULTS: After ex-posed to LPS, the expression of P2X7 receptor increased in BV-2 cells at mRNA and protein levels .The concentrations of TNF-αand IL-1βin the microglia culture supernatants also increased .Meanwhile, 0.1~10μmol/L minocycline inhibited those changes in a dose-dependent manner .CONCLUSION:Minocycline inhibits the activation of microglia .The mecha-nism may be related to the P2X7 receptor.