Changes of Kupffer cells during tree shrew chronically infected with hep-atitis B virus
10.3969/j.issn.1000-4718.2014.06.020
- VernacularTitle:树鼩慢性感染乙肝病毒过程中枯否细胞变化的意义
- Author:
Ping RUAN
;
Jian XIAO
;
Chun YANG
;
Jianjia SU
;
Chao OU
;
Ji CAO
;
Chengpiao LUO
;
Yanping TANG
;
Hong QIN
;
Wen SUN
;
Yuan LI
- Publication Type:Journal Article
- Keywords:
Tree shrews;
Hepatitis B virus;
Kupffer cells
- From:
Chinese Journal of Pathophysiology
2014;(6):1076-1081
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the changes and significance of Kupffer cells in the process of tree shrew chroni -cally infected with hepatitis B virus (HBV).METHODS:The animals were divided into 3 groups.Group A consists of 6 tree shrews that were identified as persistently infected with HBV;group B consists of 3 tree shrews that were suspected as persistently infected with HBV;group C consists of 4 tree shrews that were not inoculated with HBV and were applied as normal controls.Liver biopsies were collected regularly from all animals , and the Kupffer cells were isolated , purified and primarily cultured.The techniques of flow cytometry , immunohistochemistry, lysosomal fluorescent probe staining and real-time RT-PCR were applied to determine the number and function of these Kupffer cells .RESULTS: The result showed that the count and proportion of CD 163+cells in group A were significantly higher than those in group B and group C ( P<0.05).Meanwhile, the fluorescence intensity levels of lysosomal , the number of lysozyme-positive cells and the mRNA ex-pression level of TNF-αin the Kupffer cells in group A were significantly lower than those in group B and group C ( P<0.05).CONCLUSION:Kupffer cells may play a regulatory role during host’s chronic HBV infection.
