The protective effects of resveratral on acute radiation injury in mice
10.3760/cma.j.issn.0254-5098.2014.07.008
- VernacularTitle:白藜芦醇对急性辐射损伤小鼠的防护作用
- Author:
Hao YAN
;
Hui WANG
;
Heng ZHANG
- Publication Type:Journal Article
- Keywords:
Radiation enteritis;
Resveratrol;
Small intestinal crypt cells
- From:
Chinese Journal of Radiological Medicine and Protection
2014;34(7):512-515
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective function of resveratrol on radiation-induced small intestine injury and lethal effect in mice.Methods Mice were randomly divided into three groups:irradiation (IR) control,IR only,and IR + resveratrol.15 mice each group were irradiated on abdomen with 7.2 Gy γ-rays for cell lethal assay and 8 mice each group were irradiated with 6.5 Gy for small intestine injury assay.For the IR + resveratrol group,the mouse was given resveratrol by intragastric administration 24 h before irradiation and then was fed with resveratrol daily for 5 days.The control and IR alone groups were fed with placebo.After 30 days of IR,mouse survival rate was detected.For small intestine injury experiments,24 h after IR,the mice were terminated and the small intestines were treated with HE and immunohistochemical staining.Results Compared with the irradiation group,resveratrol increased mouse survival by 33.3%,decreased apoptosis in intestinal crypt cells (t =17.35,P < 0.05),and increased Ki67 expression (t =13.62,P < 0.05).Conclusion Resveratrol could protect small intestine injury from ionizing irradiation.