Effects of HAVCR2 gene silence on apoptosis and cell cycle in the radiation-caused genomic instable liver cells
10.3760/cma.j.issn.0254-5098.2014.07.006
- VernacularTitle:HAVCR2基因沉默对辐射诱发基因组不稳定肝细胞凋亡及细胞周期的影响
- Author:
Xuhong DANG
;
Yahui ZUO
;
Yayi YUAN
;
Biao YANG
;
Chao WANG
;
Jiangong LIU
- Publication Type:Journal Article
- Keywords:
HAVCR2 gene;
Genomic instability;
RNA interference;
Cell cycle;
Apoptosis
- From:
Chinese Journal of Radiological Medicine and Protection
2014;34(7):502-506
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of HAVCR2 siRNA on apoptosis and cell cycle in the radiation-caused genomic instable liver cells.Methods RNAi was used to inhibit HAVCR2 gene transcription.Cellular apoptosis and cell cycle distribution were detected by flow cytometry (FCM).Expression of p53 gene was assayed by real time fluorescence quantitative PCR.Results HAVCR2 gene was effectively inhibited by RNAi (t =19.21,P ＜ 0.05).After siRNA transferring,cell apoptosis (t =3.65,P ＜ 0.05) and p53 gene expression (t =4.82,P ＜ 0.05) were decreased,and G2-phase arrest was induced(t =-3.41,P ＜ 0.05).Conclusion HAVCR2 siRNA can decrease the generation of apoptosis in the genomic instable liver cells and blocks the cells at G2 phase.
