Effect of Benzalkonium, Mitomycin-C and Dexamethasone on Stress in Trabecular Meshwork Cells.
10.3341/jkos.2015.56.1.104
- Author:
Byung Moon KIM
1
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Benzalkonium chloride;
Carboxyfluorescein;
Dexamethasone;
Mitomycin C;
Trabecular meshwork cells
- MeSH:
Benzalkonium Compounds*;
Cell Survival;
Dexamethasone*;
Humans;
Mitomycin*;
Permeability;
Trabecular Meshwork*
- From:Journal of the Korean Ophthalmological Society
2015;56(1):104-108
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effects of benzalkonium chloride (BAC), mitomycin C (MMC) and dexamethasone (DEX) on cellular stress in cultured human trabecular meshwork cell (HTMC) monolayers. METHODS: HTMCs were cultured in the inner Transwell chamber until confluence and then were exposed to BAC, MMC or DEX for 6 hours. The carboxyfluorescein permeability through the HTMC monolayer was measured using a spectrofluorometer at 532 nm after 2 hours in the outer chamber. The 3-[4, 5 -dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay was used to evaluate cellular viabilities. RESULTS: The carboxyfluorescein permeability through the HTMC monolayer increased and cell survival decreased with 0.002% BAC (p < 0.05). Increased permeability without decreasing cell survival occurred with 0.05 microg/mL MMC. No effect on the permeability or cell survival was observed at 0.1 or 1.0 microm DEX (p > 0.05). CONCLUSIONS: BAC and MMC induced cellular toxicity and stress at lower concentrations but did not affect survival of cultured HTMCs.
