Effect of GPR56 on axonal development and myelination
10.3760/cma.j.issn.1671-0282.2014.06.011
- VernacularTitle:G蛋白偶联受体56参与轴突发育和髓鞘化
- Author:
Yiyu DENG
;
Gaofeng ZHOU
;
Hongke ZENG
;
Wenxin ZENG
;
Wenxin JIANG
;
Ming FANG
- Publication Type:Journal Article
- Keywords:
G-protein coupled receptor 56;
Corpus callosum;
Axon;
Myelination;
Neurofilament-200;
Proteolipid protein;
Cerebral white matter;
neuron
- From:
Chinese Journal of Emergency Medicine
2014;(6):652-657
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the likelihood of G-protein coupled receptor 56 (GPR56 ) induces axonal development and myelination in the corpus callosum of mouse brain.Methods A total of 64 Gpr56 +/-and Gpr56 -/-mice were selected and randomly divided into two groups:Gpr56 +/-group (n=32)and Gpr56 -/-group (n=32).According to number of days after birth,each group was further divided into 4 subgroups including P7d,P14d,P21d and P28d subgroups.Levels of neurofilament-200 (NF -200)and proteolipid protein (PLP ) of myelin basic protein in corpus callosum were measured with immunohistochemistry staining and Western blot in P7d、P14d、P21d、P28d Gpr56 +/- and Gpr56 -/-mice.Gpr56 +/-and Gpr56 -/-neurons were cultured using P1 d Gpr56 +/-and Gpr56 -/-mouse brain.The lengths of Gpr56 +/- and Gpr56 -/-neuronal axon were measured and compared with Image J software. Axonal myelination in the corpus callosum of mouse brain in each group was observed under electronic microscopy and the axonal diameters between subgroups were compared.Results The levels of NF-200 and PLP in the corpus callosum in P7d、P14d、P21d、P28d Gpr56 -/-mice decreased significantly compared with Gpr56 +/- mice.The length of Gpr56 -/-neuronal axon was shortened compared with Gpr56 +/-neuronal axon.The number of myelinated axons was obviously reduced in the corpus callosum in P28d Gpr56 -/-mice.The diameter of axon in the corpus callosum of P28d Gpr56 +/-mouse is longer than that of P28d Gpr56 -/-mouse. Conclusions GPR56 may be involved in axonal development and myelination in the corpus callosum of mouse brain.
