Effects of Ginkgo Biloba Extract on Learning Memory Ability and Inflammatory Response in Hippocampus of Natural Aging Mice
10.3969/j.issn.1005-5304.2014.08.014
- VernacularTitle:银杏酮酯对小鼠学习记忆能力及海马神经炎症反应的影响
- Author:
Li HAO
;
Gaiying HE
;
Zhixiong ZHANG
- Publication Type:Journal Article
- Keywords:
GBE50;
natural aging;
learning memory ability;
inflammatory response;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(8):49-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the efficiency of Ginkgo biloba extract 50 (GBE50) on learning memory ability and inflammatory response in hippocampus of natural aging mice, and explore the underlying anti-aging mechanisms.Methods 16-month-old ICR mice were randomly divided into three groups:model group, GBE50 low and high dose groups. 1 month mice were as normal group. The mice in GBE50 low and high dose groups received GBE50. The mice in the normal and model groups received solvent (1%CMC-Na+) by intragastric administration. The Morris water maze test and step-down test were used to assess behaviors of the mice. Immunofluorescent staining was used to detect the number of Iba-1 positive cells. The enzyme linked immunosorbent assay was used to determine the expression of TNF-α and IL-1β.Results Compared with normal group, escape latency and swimming distance in the Morris water maze test in the model group increased (P<0.05);latency shorted and error times decreased in the step-down test (P<0.05);the number of Iba-1 positive cell in the hippocampus CA1 in the model group increased considerably (P<0.01);TNF-α expression was in a general upward trend while IL-1β increased apparently (P<0.01). Compared with model group, escape latency and swimming distance decreased in the Morris water maze test in GBE50 high dose group;latency and the error times increased in the step-down test (P<0.05);the number of Iba-1 positive cells decreased (P<0.05). TNF-α expression showed a downward trend and IL-1β expression decreased in GBE50 low dose group (P<0.05). Conclusion GBE50 can delay aging and increase learning memory of natural aging mice.
