Content Determination of Chlorogenic Acid, Quercetin and Kaempferol inPyrrosia Lingua (Thumb.) Farwell by HPLC
10.3969/j.issn.1005-5304.2014.08.019
- VernacularTitle:HPLC同时测定石韦中绿原酸、槲皮素和山柰酚含量
- Author:
Junhua CHEN
;
Guangming ZHOU
;
Guilong PENG
;
Hongying QIN
;
Hongmei CHENG
;
Jie SHEN
- Publication Type:Journal Article
- Keywords:
Pyrrosia lingua (Thumb.) Farwell;
chlorogenic acid;
quercetin;
kaempferol;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(8):67-69
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for simultaneous separation and determination of chlorogenic acid, quercetin and kaempferol inPyrrosia lingua (Thumb.) Farwell. Methods The separation was performed on Phenomenex C18 column (250 mm×4.6 mm, 5μm) with the mobile phase of methanol-acetic acid (pH=3.0) solution and gradient elution. Flow rate was 1.0 mL/min;the UV detection wavelength was 254 nm;column temperature was 35℃.Results The calibration curves for chlorogenic acid, quercetin, and kaempferol were in good linearity in the range of 0.000 24-3.00μg (r=0.999 9), 0.000 16-2.00μg (r=0.999 9), and 0.000 16-2.00μg (r=0.999 9), respectively. The limits of detection (S/N=3) were 3.29, 0.43 and 0.33 ng/mL, respectively. The average recovery rates were 97.73%, 98.07% and 96.92%, respectively. ConclusionThe method is simple, precise and sensitive. It provides scientific proof for separation and determination of chlorogenic acid, quercetin and kaempferol inPyrrosia lingua (Thumb.) Farwell.
